ABSTRACT
Abstract Among the microorganisms that make up the intestinal microbiota, stands out Escherichia coli, which has as main ecological niche, the large human intestine. Its importance stands out in being part of the pioneer's commensal microorganisms on the colonization of the intestinal mucosa and its pathogenic role causing extra and intra intestinal diseases. The aim of the study was to evaluate the antibody production and proliferative response of Peripheral Blood Mononuclear Cells (PBMC) to E. coli antigens. The bacteria were grown on Brain Heart Infusion broth medium at 35 ºC for 72 hours. Pellet bacteria were lysed for one hour at room temperature with an 8M sodium guanidine solution. After spin and dialysis, the protein antigens were measured in the supernatant by protein assay. The antigens were characterized by polyacrylamide gel electrophoresis and the antigenic profile by western blotting. The presence of specific IgG and IgA antibodies were evaluated using thirty normal human sera by an indirect ELISA. The response of PBMC to E. coli antigens was assessed by MTT metabolization. The results demonstrated that the antigens were composed of proteins of different sizes and they were recognized by antibodies present in normal human serum. Human sera presented high titers of IgG and IgA antibodies to E. coli antigens when compared to the results of lipopolysaccharide. We also showed that total E. coli antigens induced PBMC proliferation at different antigen concentrations. Taken together the results suggest that the antigens from E. coli can induce local and systemic immune responses.
ABSTRACT
Os produtos embutidos estão sendo cada vez mais consumidos pela população, devido sua praticidade e durabilidade, além disso, serem muito saborosos. Visando a saúde pública, todos os alimentos devem passar por uma rigorosa inspeção, para garantir que o alimento chegue à mesa do brasileiro com toda garantia e qualidade. Foram pesquisadas cinco marcas de salames provenientes de supermercados e feiras livres da cidade de Alfenas MG, analisadas em duplicata, com duas repetições, sendo essas repetições feitas em intervalos e lotes diferentes para observação da qualidade do produto. Os dados obtidos foram surpreendentes, onde 60% das amostras analisadas apresentaram-se impróprias para o consumo por albergarem Salmonella sp.
Subject(s)
Animals , Cattle , Food Contamination/analysis , Food Inspection , Industrialized Foods , Food Microbiology , Meat Products/microbiology , Salmonella/isolation & purification , Swine , Brazil , Food Samples , Listeria monocytogenes/isolation & purificationABSTRACT
A planta Plectranthus ornatus Codd é originária dos países do Mediterrâneo e Oriente Próximo. Na medicina popular é indicada para males do fígado e problemas de digestão. É utilizada no tratamento para o controle da gastrite, na dispepsia, azia, mal-estar gástrico e ressaca. Este trabalho objetiva a avaliação antimicrobiana do extrato de P. ornatus, utilizando-se 15 microrganismos padronizados. Os testes antimicrobianos foram realizados em ágar Mueller Hinton pela técnica de poços. Foram utilizados os testes de microdiluição em caldo, para a determinação da concentração inibitória mínima (CIM) e semeadura, em placas contendo ágar Mueller Hinton para a concentração microbicida mínima (CMM). Os testes de microdiluição em caldo demonstraram que o extrato nas concentrações de 20,31; 325 e 650 mg/mL inibiu o crescimento bacterianode Bacillus cereus, Streptococcus pyogenes e Enterococcus faecalis, respectivamente. Para o fungo Saccharomyces cerevisiae os testes de microdiluição demonstraram inibi- ção do crescimento na concentração de 1.300 mg/mL. Para as demais cepas testadas, o extrato não demonstrou atividade. Em decorrência da crescente resistência múltipla microbiana aos antibióticos, pesquisas para o desenvolvimento de novos medicamentosque sejam economicamente viáveis e com margem de segurança efetiva têm ganhado espaço na comunidade científica.
Plectranthus ornatus Codd is a plant from Mediterranean and Near East countries. It is indicated for liver illnesses and digestion problems in popular medicine. It is used in the treatment for the control of gastritis, dyspepsia, heartburn, gastric discomfort, and hangover. This study aims to evaluate the antimicrobial activity in extracts of P. ornatus using 15 standardized micro-organisms. Antimicrobial tests were performed on Mueller Hinton agar through the technique of wells. Broth microdilution tests were used for the determination of the minimum inhibitory concentration (MIC), and seeding on plates containing Mueller Hinton agar for the determination of the minimum microbicide concentration (MMC). The broth microdilution tests demonstrated that the extract inhibited the growth of Bacillus cereus, Streptococcus pyogenes, and Enterococcus faecalis at the concentrations of 20.31, 325, and 650 mg/mL, respectively. The microdilution tests showed growth inhibition of Saccharomyces cerevisiae at a concentration of 1,300 mg/mL. The extract did not show inhibitory activity on the other tested strains. As a result of increased multiple resistances to antibiotics,microbial research in the development of new drugs that are economically viable and offer an effective safety margin have won space in the scientific community.